Inhibition of quorum sensing (QS) in Yersinia enterocolitica by an orange extract rich in glycosylated flavanones.
Identifieur interne : 001272 ( Main/Exploration ); précédent : 001271; suivant : 001273Inhibition of quorum sensing (QS) in Yersinia enterocolitica by an orange extract rich in glycosylated flavanones.
Auteurs : Pilar Truchado [Espagne] ; Juan-Antonio Giménez-Bastida ; Mar Larrosa ; Irene Castro-Ibá Ez ; Juan Carlos Espín ; Francisco A. Tomás-Barberán ; María Teresa García-Conesa ; Ana AllendeSource :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2012.
English descriptors
- KwdEn :
- Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Citrus sinensis (chemistry), Down-Regulation (drug effects), Flavanones (chemistry), Flavanones (pharmacology), Gene Expression Regulation, Bacterial (drug effects), Glycosylation, Humans, Plant Extracts (chemistry), Plant Extracts (pharmacology), Quorum Sensing (drug effects), Yersinia Infections (microbiology), Yersinia enterocolitica (drug effects), Yersinia enterocolitica (genetics), Yersinia enterocolitica (physiology).
- MESH :
- chemical , chemistry : Flavanones, Plant Extracts.
- chemical , genetics : Bacterial Proteins.
- chemical , metabolism : Bacterial Proteins.
- chemistry : Citrus sinensis.
- drug effects : Down-Regulation, Gene Expression Regulation, Bacterial, Quorum Sensing, Yersinia enterocolitica.
- genetics : Yersinia enterocolitica.
- microbiology : Yersinia Infections.
- chemical , pharmacology : Flavanones, Plant Extracts.
- physiology : Yersinia enterocolitica.
- Glycosylation, Humans.
Abstract
Flavanones, flavonoids abundant in Citrus , have been shown to interfere with quorum sensing (QS) and affect related physiological processes. We have investigated the QS-inhibitory effects of an orange extract enriched in O-glycosylated flavanones (mainly naringin, neohesperidin, and hesperidin). The QS-inhibitory capacity of this extract and its main flavanone components was first screened using the bacteriological monitoring system Chromobacterium violaceum . We next examined the ability of the orange extract and of some of the flavanones to (i) reduce the levels of the QS mediators produced by Y. enterocolitica using HPLC-MS/MS, (ii) inhibit biofilm formation, and (iii) inhibit swimming and swarming motility. Additionally, we evaluated changes in the expression of specific genes involved in the synthesis of the lactones (yenI, yenR) and in the flagellar regulon (flhDC, fleB, fliA) by RT-PCR. The results showed that the orange extract and its main flavanone components inhibited QS in C. violaceum, diminished the levels of lactones secreted by Y. enterocolitica to the media, and decreased QS-associated biofilm maturation without affecting bacterial growth. Among the tested compounds, naringin was found to inhibit swimming motility. Exposure to the orange extract and (or) to naringin was also found to be associated with induction of the transcription levels of yenR, flhDC, and fliA. This work shows the in vitro QS-inhibitory effects of an orange extract enriched in flavanones against a human enteropathogen at doses that can be achieved through the diet and suggests that consumption of these natural extracts may have a beneficial antipathogenic effect.
DOI: 10.1021/jf301365a
PubMed: 22533445
Affiliations:
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Le document en format XML
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Box 164, 30100 Campus de Espinardo, Murcia, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC , P.O. 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Tomás-Barberán</name></author><author><name sortKey="Garcia Conesa, Maria Teresa" sort="Garcia Conesa, Maria Teresa" uniqKey="Garcia Conesa M" first="María Teresa" last="García-Conesa">María Teresa García-Conesa</name></author><author><name sortKey="Allende, Ana" sort="Allende, Ana" uniqKey="Allende A" first="Ana" last="Allende">Ana Allende</name></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="eISSN">1520-5118</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (metabolism)</term><term>Citrus sinensis (chemistry)</term><term>Down-Regulation (drug effects)</term><term>Flavanones (chemistry)</term><term>Flavanones (pharmacology)</term><term>Gene Expression Regulation, Bacterial (drug effects)</term><term>Glycosylation</term><term>Humans</term><term>Plant Extracts (chemistry)</term><term>Plant Extracts (pharmacology)</term><term>Quorum Sensing (drug effects)</term><term>Yersinia Infections (microbiology)</term><term>Yersinia enterocolitica (drug effects)</term><term>Yersinia enterocolitica (genetics)</term><term>Yersinia enterocolitica (physiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Flavanones</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Down-Regulation</term><term>Gene Expression Regulation, Bacterial</term><term>Quorum Sensing</term><term>Yersinia enterocolitica</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Yersinia enterocolitica</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Yersinia Infections</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Flavanones</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Yersinia enterocolitica</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Glycosylation</term><term>Humans</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Flavanones, flavonoids abundant in Citrus , have been shown to interfere with quorum sensing (QS) and affect related physiological processes. We have investigated the QS-inhibitory effects of an orange extract enriched in O-glycosylated flavanones (mainly naringin, neohesperidin, and hesperidin). The QS-inhibitory capacity of this extract and its main flavanone components was first screened using the bacteriological monitoring system Chromobacterium violaceum . We next examined the ability of the orange extract and of some of the flavanones to (i) reduce the levels of the QS mediators produced by Y. enterocolitica using HPLC-MS/MS, (ii) inhibit biofilm formation, and (iii) inhibit swimming and swarming motility. Additionally, we evaluated changes in the expression of specific genes involved in the synthesis of the lactones (yenI, yenR) and in the flagellar regulon (flhDC, fleB, fliA) by RT-PCR. The results showed that the orange extract and its main flavanone components inhibited QS in C. violaceum, diminished the levels of lactones secreted by Y. enterocolitica to the media, and decreased QS-associated biofilm maturation without affecting bacterial growth. Among the tested compounds, naringin was found to inhibit swimming motility. Exposure to the orange extract and (or) to naringin was also found to be associated with induction of the transcription levels of yenR, flhDC, and fliA. This work shows the in vitro QS-inhibitory effects of an orange extract enriched in flavanones against a human enteropathogen at doses that can be achieved through the diet and suggests that consumption of these natural extracts may have a beneficial antipathogenic effect.</div></front></TEI><affiliations><list><country><li>Espagne</li></country></list><tree><noCountry><name sortKey="Allende, Ana" sort="Allende, Ana" uniqKey="Allende A" first="Ana" last="Allende">Ana Allende</name><name sortKey="Castro Iba Ez, Irene" sort="Castro Iba Ez, Irene" uniqKey="Castro Iba Ez I" first="Irene" last="Castro-Ibá Ez">Irene Castro-Ibá Ez</name><name sortKey="Espin, Juan Carlos" sort="Espin, Juan Carlos" uniqKey="Espin J" first="Juan Carlos" last="Espín">Juan Carlos Espín</name><name sortKey="Garcia Conesa, Maria Teresa" sort="Garcia Conesa, Maria Teresa" uniqKey="Garcia Conesa M" first="María Teresa" last="García-Conesa">María Teresa García-Conesa</name><name sortKey="Gimenez Bastida, Juan Antonio" sort="Gimenez Bastida, Juan Antonio" uniqKey="Gimenez Bastida J" first="Juan-Antonio" last="Giménez-Bastida">Juan-Antonio Giménez-Bastida</name><name sortKey="Larrosa, Mar" sort="Larrosa, Mar" uniqKey="Larrosa M" first="Mar" last="Larrosa">Mar Larrosa</name><name sortKey="Tomas Barberan, Francisco A" sort="Tomas Barberan, Francisco A" uniqKey="Tomas Barberan F" first="Francisco A" last="Tomás-Barberán">Francisco A. Tomás-Barberán</name></noCountry><country name="Espagne"><noRegion><name sortKey="Truchado, Pilar" sort="Truchado, Pilar" uniqKey="Truchado P" first="Pilar" last="Truchado">Pilar Truchado</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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